Molecular Characterization of Induced Mutagenesis Through Diethyl Sulfate using SRAP Markers for Improvement of Morphological Characters in Gypsophila elegans M. Bieb Plants

Radwan, R.M.S; Eid, Rawia A.; Abd El-Aziz, Nahed G.; Mahfouze, Heba A.; Ashour, Hossam; El-Khateeb, M. A.

doi:10.21608/jassd.2025.369295.1044

Molecular Characterization of Induced Mutagenesis Through Diethyl Sulfate using SRAP Markers for Improvement of Morphological Characters in Gypsophila elegans M. Bieb Plants

Document Type : Original Article

Authors

¹ Department of Ornamental Plants and Woody Trees Agricultural and Biological Research Institute, National Research Centre Dokki, Giza, Egypt

² Ornamental plants and woody trees department, Agricultural and biological research institute, National research center, Dokki, Giza, Egypt

³ Ornamental Plants and Woody Trees Department, Institute of Agricultural and Biological Research’s, National Research Centre, Dokki, Cairo, Egypt

⁴ Genetics and Cytology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt

⁵ Department of Ornamental Hort., Faculty of Agric., Cairo University, Egypt

⁶ Department of Ornamental Hort., Faculty of Agric., Cairo University, Egypt.

10.21608/jassd.2025.369295.1044

Abstract

Biotechnological techniques that are related to classical breeding methods have been applied for modifying the morphological characteristics. So, diethyl sulphate was used to induce genetic variability in G. elegans plant to improve morphological characters. Besides, the changes in the genomic DNA between mutant plants and the control were investigated. This study was performed by soaking the seeds in four different DES concentrations from (1000 - 4000 ppm) for 8 h. The results revealed that untreated plants gave the highest germination percentage in M1 and M2 respectively, whilst the lowest percentage was achieved with 4000 ppm. Using DES at 1000 and 2000 ppm DES in M1 and M2, respectively, induced significant increases in the plant height, branches formation, flowers production, and caused early flowering compared to the control, and with increasing DES concentration to 4000 ppm decreased these characters to the minimum values, but increased number of days elapsed to flowering. Many mutants in leaves and flowers morphology were recorded; the highest number of these mutants was observed with 4000 ppm. Molecular marker analyses using SRAP markers, showed that the largest number of bands was produced by primer SRAP-4 (8 bands), while the lowest number was generated by primer SRAP-6 (3 bands). Whilst, primer SRAP-2 displayed the highest number of polymorphism (66.67%), but SRAP-4 scored the lowest number of polymorphism (12.50%). Therefore, it was concluded that, using SRAP markers, the existence of genetic diversity at the genomic DNA level between mutants DES-treated and control, depending on DES concentration, has been confirmed.

Keywords